Combinatorial peptide ligand library plasma treatment: Advantages for accessing low-abundance proteins
نویسندگان
چکیده
منابع مشابه
Combinatorial peptide libraries facilitate development of multiple reaction monitoring assays for low-abundance proteins.
Low-abundance proteins present in biological fluids are often considered an attractive source of new disease biomarkers. Since such proteins are poorly observed in proteome-scale discovery experiments due to an overwhelming mass of high-abundance proteins, the development of quantitative multiple reaction monitoring (MRM) assays for low-abundance proteins is a challenging task. Here, we present...
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Introduction Biomarker discovery is one of the driving forces of proteomics programs in the life sciences. The utility of biomarkers as the biophysical or biochemical measurements and indicators of disease status or response to therapy is established in clinical research. However, an increased number of validated markers is needed, and advances in genomics and proteomics research have opened th...
متن کاملHexapeptide combinatorial ligand libraries: the march for the detection of the low-abundance proteome continues.
After 10 years of extensive proteomic research, it has become increasingly apparent that new technologies are sorely needed for detecting the low-abundance proteome-those proteins (up to 50% in any proteome) whose concentration in tissues or cells falls below the detection limits of currently available methodologies. Here we survey one such method: a combinatorial ligand library (called ProteoM...
متن کاملHigh Abundance Proteins Depletion vs Low Abundance Proteins Enrichment: Comparison of Methods to Reduce the Plasma Proteome Complexity
BACKGROUND To date, the complexity of the plasma proteome exceeds the analytical capacity of conventional approaches to isolate lower abundance proteins that may prove to be informative biomarkers. Only complex multistep separation strategies have been able to detect a substantial number of low abundance proteins (<100 ng/ml). The first step of these protocols is generally the depletion of high...
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N-acetyl glucosamine binding protein amplifies the concentration of one member in a dynamic combinatorial glycopeptide library based on exchanging disulfides.
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ژورنال
عنوان ژورنال: PROTEOMICS - Clinical Applications
سال: 2010
ISSN: 1862-8346
DOI: 10.1002/prca.201090092